GingaNinga

I have this problem with a bunch of new hires. I’ll show them another way to do something and they’ll ask, oh where was that written down? I said Just think about what I just did and how it makes sense, its not written down this is a neat trick i’m showing you. I swear there is no creativity or critical thinking anymore, just a bunch of automatons that follow protocol to the letter and the second there is a situation outside those very narrow parameters they just implode. Someone had to figure all of this out at one point and make the protocol in the first place, sometimes there is no step by step guide and you need to exercise judgement and make some decisions on your own.

slam

suppository

I like everything about the PS5 better: I LOVE the controller, UI, games play awesome on the machine and in general I prefer their more story-entered games BUT PS+ straight up sucks, there’s nothing on there for me and its outrageously priced. Xbox on the other hand still runs great, everything about the controller and UI is just fine but game pass rules. Its also overpriced but at least they have a massive catalogue of games I actually want to try.

I don’t think you can do that with a single population of cells. Theres a group of cells that sample antigens and process them for presentation then a separate group of cells that the first group will present the antigen to. Once presented there are a set of standardized regions related to the class of antibody then a set of “hypervariable” regions. You would need an organoid/organ system to accomplish this in vitro or ex vivo. Most of the time it will be a macrophage or something that for example ingests a live bacteria (or venom), it will register this as foreign, process the particle and travel to a lymph node where all the immunology takes place (B cells). I don’t think theres a simple way to recreate this in the lab its completely different from synthesizing chemicals since each antibody is fine-tuned to its antigen. You might even get different antibodies generated for the same compound depending on how everything went down and which region was presented (eg monoclonal vs poloclonal products).

Thanks for the explainer! My lab specializes in IHC so I’m generally familiar with ag-ab interactions but not so much with antibody development. Its fascinating!

To my knowledge its easy to do this with proteins that are directly coded for in our genes. For example there is a gene for insulin so we can clip it, transfect it into a cell culture and get those cells to crank out a bunch of insulin that we can extract, purify and make into a usable, stable product. The problem with antibodies is that we need to be exposed to an antigen capable of stimulating the development of the corresponding antibody. There is a very complicated set of receptors that capture the antigen, internalize and process it to present it to cells that will modify a whole bunch stuff (Thats the limit of my personal knowledge) to generate an antibody capable of neutralizing that substance. In short you still need a living being with some sort of immune system capable of processing that compound and generating an antibody to it, its unbelievably complicated. The good news is once that cell starts cranking out antibody it starts to clone itself so we can extract it, make a hybridoma (fuze the antibody-producing cell from a live animal with immortal cancer cells to create an immortal hybrid that cranks out the desired antibody) then we can continue with the process in cell culture as per usual. Its just cheaper and easier to use live animals with high titre to that antibody. Source: I’ve taken a couple immunology courses and my lab specializes in immunohistochemistry so I’m quite familiar with antigen/antibody interactions but not so much with antibody production.

The immune system is super complicated but essentially when an animal or person are exposed to some sort of substance white blood cells sample that compound and create an antibody that is tailored to bind and neutralize it. Different substances range in their ability to induce this response and it often takes repeat exposures to get the titre to high enough levels. So one way or another some sort of animal needs to be exposed to the venom whether it be horse, goat or mouse and we would harvest the antibody for use in emergency situations where we need to immediately reverse the effects of a snake bite.

Another way would be to do this in cell culture but I’m not quite sure how that works, I think thats easier to do when the proteins are directly coded for like insulin or something. You could probably harvest B cell clones that produce the antibody to the venom, make them immortal and harvest it that way but I’m less familiar with that sort of wok so I can’t really comment further.

slam

what about switch 2

I bet it was Arby’s, they always claim to have “the meats”.

no worries, nice catch!

I can’t remember the last time a movie came out that made me want to go see in theatres. Tickets are so expensive that I only want to go to one or two movies a year. Then with TV I find every show these days has “netflix syndrome” with lazy writing, exposition dumps, dummed down dialogue, I’m just not interested in what they have to offer most of the time.

ahh member skype?

I use it for school. I’m in different groups and I need to use it for chat, file and image sharing on different projects and its an absolute nightmare trying to keep track of channels, I’d rather use literally any other software to accomplish this task. Most of us just jump on discord and use that.

I cant stand anything microsoft anymore. Teams, outlook, word, every iteration just makes me more angry.

Ya Engineering really is 't my thing but it seams like such a logical way to do things. This would even be great with phones if you could just swap out the battery you’re not currently using.

I wish the batteries were modular/interchangeable. You could just pull into a station, remove the spent battery and replace it with a full one, the spent one can then just get recharged and stored at the station for the next user to change out. You could even bring some extra ones in the trunk for a long trip!

I don’t want that on my phone. I just want a dumb program that sets alerts and schedules, I don’t want it interpreting information and I doubt most people really need that function on a phone.

every NVDA earnings call lol. Old man Jenson had a (chip) farm, AI AI OH! guy literally said AI almost 100 times in a call.